Extraction of beta-carotene from carrot via soxhlet extraction method

Mohamad Sazwan, Ismail (2013) Extraction of beta-carotene from carrot via soxhlet extraction method. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.

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Abstract

Extraction of carotene from carrot (Daucus Carota.L) using Soxhlet extraction method was done by using different solvent and time. Beta-carotene is the highest constituents in carotene. Fresh carrots are used as sample in this study. The effect of different parameters, which are type of solvents and duration of extraction time. Three types of solvents are used in this research which are acetone, ethanol, and 2-propanol. While, the duration of extraction time used are 2, 4, 6, and 8 hours. The overall process of this research involves four major steps which are sample preparation, extraction of carotene compound by using Soxhlet extraction, solvent separation of extracted compound by using rotary evaporator and analysis of carotene yield by using HPLC. When separated the desired compound from solvent, set the evaporator temperature at the boiling point of the solvent. The samples is then need to be filter first for the purpose of HPLC. The LC-18 is use as our HPLC column. The peak area for acetone is 19.7714%, 11.7953% for ethanol and 11.9622% for 2-propanol. The optimum extraction time for acetone is 6 hours. So, as a nutshell acetone is the best solvent with the optimum extraction time of 6 hours

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2013; SV:DR. HANUNI BINTI RAMLI@SAID; CD:8324
Uncontrolled Keywords: Extraction (Chemistry)
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mr. Syed Mohd Faiz Syed Abdul Aziz
Date Deposited: 15 Oct 2015 06:15
Last Modified: 16 Nov 2023 07:04
URI: http://umpir.ump.edu.my/id/eprint/10771
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