Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support

Ponnarasy, Ganasen and Khan, Maksudur R. and M., Abul Kalam and Mohd Sabri, Mahmud (2014) Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support. Bioprocess and Biosystems Engineering, 37 (11). pp. 2353-2359. ISSN 1615-7591 (print); 1615-7605 (online). (Published)

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DOI/Official URL: http://dx.doi.org/10.1007/s00449-014-1213-6

Abstract

This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.

Item Type:	Article
Additional Information:	Indexed in Scopus
Uncontrolled Keywords:	Light irradiation; Hydrolysis; Pseudomonas cepacia; p-Nitrophenyl palmitate
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) T Technology > TP Chemical technology
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering
Depositing User:	Ms. Nurul Amanina Lokman
Date Deposited:	22 Mar 2016 03:21
Last Modified:	17 Aug 2017 03:07
URI:	http://umpir.ump.edu.my/id/eprint/12375
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