Tony, Hadibarata and Khudhair, Ameer Badr and Kristanti, Risky Ayu and Kamyab, Hesam (2017) Biodegradation of Pyrene by Candida sp. S1 under High Salinity Conditions. Bioprocess and Biosystems Engineering, 40 (9). pp. 1411-1418. ISSN 1615-7591. (Published)
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Abstract
Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L-1 of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5. It was also found that the increasing glucose concentration of more than 10 g L-1 had no significant effect on pyrene biodegradation, while agitation proved to have greater influence. There was a positive relationship between biomass growth and biodegradation rate of pyrene. One pyrene metabolite was identified from the extract solution and analyzed by a thinlayer chromatography, UV–visible absorption and gas chromatography–mass spectrometry. The metabolite found in the pyrene degradation was benzoic acid.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexes in Scopus. IF: 1.870 |
| Uncontrolled Keywords: | Candida sp. S1; Pyrene biodegradation; Metabolite identification; Salinity |
| Subjects: | T Technology > T Technology (General) |
| Faculty/Division: | Faculty of Engineering Technology |
| Depositing User: | Mrs. Neng Sury Sulaiman |
| Date Deposited: | 11 Jul 2017 04:21 |
| Last Modified: | 15 Jan 2018 07:26 |
| URI: | http://umpir.ump.edu.my/id/eprint/18099 |
| Download Statistic: | View Download Statistics |
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