Mimi Sakinah, A. M. and Noor Suhana, Adzahar (2011) Application of Fractional Factorial Design (FFD) for Screening of Significant Factor in Influencing Succinic acid Production from Biodiesel Based Glycerol Using Escherichia Coli. In: 4th International Conference on Modeling, Simulation and Applied Optimization (ICMSAO) 2011 , 19-21 April 2011 , Kuala Lumpur. pp. 1-5..
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Abstract
The production of succinic acid from biodiesel based glycerol was carried out using Escherichia coli K12 MG1655 strain. The contribution of every parameter was evaluated and optimized using statistical modeling. A 2 6-1 fractional factorial design (FFD) was applied to screen the effect of substrate concentration, tryptone concentration, sodium carbonate concentration, inoculums density, pH and incubation period for Escherichia coli reaction on succinic acid production. The result of first order factorial design showed that glycerol concentration, tryptone concentration, incubation period and pH had significant positive effect (p>0.001) to the reaction. Meanwhile, inoculums density and Na2CO concentration exhibited insignificant effect in the reaction. In addition, interaction between inoculums density of substrate concentration and tryptone concentration, glycerol concentration and Na23CO concentration, tryptone concentration and pH, Na32CO concentration and incubation period and inoculums density with pH gave significant effects (p<0.001) to succinic acid production.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Additional Information: | Please click to official URL |
| Uncontrolled Keywords: | Glycerol; Escherichia coli; statistical model; Fractional Factorial design (FFD) |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Pn. Hazlinda Abd Rahman |
| Date Deposited: | 29 Dec 2011 05:55 |
| Last Modified: | 17 Jan 2018 06:35 |
| URI: | http://umpir.ump.edu.my/id/eprint/2026 |
| Download Statistic: | View Download Statistics |
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