He, Yuhai and Syed Mohd Saufi, Tuan Chik and Chong, Fui Chin (2014) Application of Adsorption Chromatography in the Papain Processing. GSTF Journal of Chemical Sciences (JChem), 1 (2). pp. 9-15. ISSN 2339-5079 (Online). (Published)
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Abstract
A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Papain, Chromatography, RP-EBAC, Purification |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Mrs. Neng Sury Sulaiman |
| Date Deposited: | 18 Feb 2016 06:00 |
| Last Modified: | 26 Mar 2018 07:33 |
| URI: | http://umpir.ump.edu.my/id/eprint/6260 |
| Download Statistic: | View Download Statistics |
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