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Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)

Alinejad, T. and Kwong, Q. Bin and Jaya Vejayan, Palliah and Othman, R. Y. and Bhassu, S. (2015) Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). Meta Gene, 5 (Sept.). pp. 55-67. ISSN 2214-5400

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Abstract

Epizootic diseases cause huge mortality and economical loses at post larvae stages in freshwater prawn aquaculture industry. These prawns seem less susceptible to viral diseases except for infectious hypodermal and hematopoietic necrosis virus (IHHNV). During viral infection in prawns, hemocytes are the primary organ that shows immunological response within the early stages of infection. We applied proteomic approaches to understand differential expression of the proteins in hemocytes during the viral disease outbreak. To aid the goal, we collected Macrobrachium rosenbergii broodstocks from the local grow out hatchery which reported the first incidence of IHHNV viral outbreak during larvae stage. Primarily, application of the OIE primer targeting 389 bp fragments of IHHNV virus was used in identification of the infected and non-infected samples of the prawn breeding line. Analysis of two-dimensional gel electrophoresis showed specific down-regulation of Arginine kinase and Sarcoplasmic calcium-binding protein and up/down-regulation of Prophenoloxidase1 and hemocyanin isoforms. These proteins were validated using semi quantitative RT-PCR and gene transcripts at mRNA level. These identified proteins can be used as biomarkers, providing a powerful approach to better understanding of the immunity pathway of viral disease with applications in analytic and observational epidemiology diagnosis. Proteomic profiling allows deep insight into the pathogenesis of IHHNV molecular regulation and mechanism of hemocyte in freshwater prawns

Item Type: Article
Uncontrolled Keywords: Hemocyte; IHHNV virus; Differentially expressed proteins; Proteomics; Genomics
Subjects: Q Science > QP Physiology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Noorul Farina Arifin
Date Deposited: 04 Apr 2016 06:29
Last Modified: 21 Feb 2018 06:55
URI: http://umpir.ump.edu.my/id/eprint/12636
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