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A Comprehensive Review on l-Asparaginase and Its Applications

Batool, Tahira and Makky, Essam A. and Ali, Muna Jalal and M. M., Yusoff (2016) A Comprehensive Review on l-Asparaginase and Its Applications. Applied Biochemistry and Biotechnology, 178 (5). pp. 900-923. ISSN 1559-0291

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Abstract

l-asparaginase (LA) catalyzes the degradation of asparagine, an essential amino acid for leukemic cells, into ammonia and aspartate. Owing to its ability to inhibit protein biosynthesis in lymphoblasts, LA is used to treat acute lymphoblastic leukemia (ALL). Different isozymes of this enzyme have been isolated from a wide range of organisms, including plants and terrestrial and marine microorganisms. Pieces of information about the three-dimensional structure of l-asparaginase from Escherichia coli and Erwinia sp. have identified residues that are essential for catalytic activity. This review catalogues the major sources of l-asparaginase, the methods of its production through the solid state (SSF) and submerged (SmF) fermentation, purification, and characterization as well as its biological roles. In the same breath, this article explores both the past and present applications of this important enzyme and discusses its future prospects.

Item Type: Article
Uncontrolled Keywords: L-asparaginase; Lymphoblasts; Acute lymphoblastic leukemia; Solid state fermentation; Submerged fermentation
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Dr. Essam A. Makky
Date Deposited: 07 Apr 2016 08:07
Last Modified: 26 Jul 2018 06:50
URI: http://umpir.ump.edu.my/id/eprint/12747
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