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Uric Acid Detection in UV Region

Nor Mazlee, Norazmi and Hadi, Manap and Zul Rasyied, Abdul Rasat and Mazuina, Mohamad (2016) Uric Acid Detection in UV Region. In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 752-757..

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Abstract

The aim of this research is to analyze uric acid (UA) concentration using Ultraviolet (UV) spectrometer. Absorption technique was proposed in the application to detect different uric acid concentrations. Current practice is commonly done by using enzymatic or colorimetric technique which may drag take a lot of times especially at the analysis phase. By this proposed spectroscopic technique, every concentration can be contemplated into UV absorption wavelength displaying on Spectrasuite. It shows fast time response i.e., 1s. Each value of diluted uric acid concentration indicates a different absorption potential. Absorption cross-section of uric acid can be calculated by Beer’s Lambert law formula. The experiment proves that different concentrations of uric acid were successfully detected using UV spectrometer at wavelength, 294.46 nm, every concentration showing a different absorbance value. Meanwhile, the absorption cross-section of uric acid molecules shows the close values for the different concentrations.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Uric acid, Ultraviolet (UV), UV absorption wavelength, Concentration
Subjects: Q Science > QC Physics
Faculty/Division: Faculty of Engineering Technology
Depositing User: Rosfadilla Mohamad Zainun
Date Deposited: 20 Dec 2016 01:19
Last Modified: 16 Jan 2020 03:19
URI: http://umpir.ump.edu.my/id/eprint/15762
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