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Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells

Swee, Keong Yeap and Nadiah, Abu and Akhtar, Muhammad Nadeem and Wan, Yong Ho and Huynh, Ky and Sheau, Wei Tan and Noorjahan Banu, Alitheen and Tunku, Kamarul (2017) Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. Integrative Cancer Therapies, 16 (3). pp. 373-384. ISSN 1534-7354 (print); 1552-695X (online)

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Abstract

Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2–induced cell death is via neutralization of reactive oxygen species.

Item Type: Article
Uncontrolled Keywords: flavokawain B, HeLa, cervical cancer, antioxidant, iron sequestration
Subjects: Q Science > QD Chemistry
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Noorul Farina Arifin
Date Deposited: 13 Sep 2017 06:12
Last Modified: 13 Sep 2017 06:12
URI: http://umpir.ump.edu.my/id/eprint/16811
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