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Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells

Norsharina, Ismail and Akhtar, Muhammad Nadeem and Maznah, Ismail and Seema, Zareen and Syed Adnan, Ali Shah and Nordin, Hj Lajis and Saiful Nizam, Tajuddin (2015) Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells. Natural Product Research: Formerly Natural Product Letters, 29 (16). pp. 1571-1574. ISSN 1478-6419 (print); 1478-6427 (online)

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Abstract

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects.

Item Type: Article
Uncontrolled Keywords: neuroprotective; Knema laurina; Hydrogen peroxide; Aβ1 – 42; SH-SY5Y cell; Flavonoids
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 02 Mar 2017 04:11
Last Modified: 19 Apr 2018 02:34
URI: http://umpir.ump.edu.my/id/eprint/16897
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