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Synthetic Curcumin Derivative DK1 Possessed G2/M Arrest and Induced Apoptosis Through Accumulation of Intracellular ROS in MCF-7 Breast Cancer Cells

Norlaily, Mohd Ali and Swee, Keong Yeap and Nadiah, Abu and Kian, Lam Lim and Huynh, Ky and Ahmad Zaim, Mat Pauzi and Wan, Yong Ho and Sheau, Wei Tan and Han, Kiat Alan-Ong and Seema, Zareen and Noorjahan Banu, Alitheen and Akhtar, Muhammad Nadeem (2017) Synthetic Curcumin Derivative DK1 Possessed G2/M Arrest and Induced Apoptosis Through Accumulation of Intracellular ROS in MCF-7 Breast Cancer Cells. Cancer Cell International, 17 (30). pp. 1-12. ISSN 1475-2867(print); 1475-2867(online)

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Abstract

Curcumin is a lead compound of the rhizomes of Curcuma longa and possess a broad range of pharmacological activities. Chemically, curcumin is 1,3-dicarbonyl class of compound, which exhibits keto-enol tautomerism. Despite of its strong biological properties, curcumin has yet been recommended as a therapeutic agent because of its poor bioavailability. A curcumin derivative (Z)-3-hydroxy-1-(2-hydroxyphenyl)-3-phenylprop-2-en-1-one (DK1) was synthesized and its cytotoxicity was tested on breast cancer cell MCF-7 and normal cell MCF-10A using MTT assay. Meanwhile, cell cycle regulation and apoptosis on MCF-7 cell were evaluated using flow cytometry. Regulation of cell cycle and apoptosis related genes expression was investigated by quantitative real time polymerase chain reaction (qRT-PCR), western blot and caspases activity analyses. Activation of oxidative stress on MCF-7 were evaluated by measuring ROS and GSH levels.

Item Type: Article
Uncontrolled Keywords: DK1; ROS; Apoptosis; Cell cycle arrest; CDC2 phosphorylation
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 19 Jul 2017 04:12
Last Modified: 29 Dec 2017 05:05
URI: http://umpir.ump.edu.my/id/eprint/17120
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