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Waste Management Biotechnology for Alkaline Protease Production and Optimization

Thanoon, Raid D. and Rubaaini, Subramaniam and Mahmood, Mahmood H. and Munem, Osama A. and Makky, Essam A. and M. M., Yusoff (2017) Waste Management Biotechnology for Alkaline Protease Production and Optimization. In: Proceedings of the FGIC 1st Conference on Governance & Integrity, 3-4 April 2017 , Yayasan Pahang, Kuantan, Malaysia. pp. 308-322.. ISBN 978-967-2054-37-5

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Abstract

Environmental pollution is one of the major issues in either well developed or in developing countries. Pollution can also come from animal wastes such as livestock, wastewater and feed that have been wasted. This type of circumstances occurs when there are abundant of waste generated in animal farms in certain area that is difficult to be disposed. For example, the fish residues such as fish fin, viscera, and scales can be one of the sources of contamination that can be the major problem. Moreover, chicken feather (CF) is widely disposed that results in environmental pollution as it cannot be solved with ease. Hence both fish fin (FF) and CF can be utilized to produce alkaline protease (AP) enzyme by using microbial fermentation power. Protein from each sample was extracted and determined. Then, the extracted protein from both wastes was employed in the production media together with bacterial suspension which was incubated. Next, alkaline protease assay was carried out to test for presence of alkaline protease in the samples. Optimization parameters controlling alkaline protease productivity were screened and determined. The optimum pH of CF and FF was 9.0, the optimum temperatures recorded at 28 and 40°C for CF and FF respectively, the production incubation periods were 6 and 10 days for CF and FF respectively. While, galactose and glucose exhibited the optimum carbon sources for CF and FF respectively from one hand, and the optimum nitrogen sources recorded to be ammonium chloride and beef extract for CF and FF respectively from the other hand.

Item Type: Conference or Workshop Item (Speech)
Uncontrolled Keywords: Alkaline Protease; Fish fin; Chicken feather; Waste management; Protein extraction; Optimization
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Pn. Hazlinda Abd Rahman
Date Deposited: 12 May 2017 06:32
Last Modified: 27 Jul 2018 08:30
URI: http://umpir.ump.edu.my/id/eprint/17619
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