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Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques

Muhammad Fuad, Abdullah (2010) Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.

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Abstract

Variety of proteins exists in bovine whey such as a-lactalbumin (a-lac), β-lactoglobulin, (β-lg), bovine serum albumin (BSA) and immunoglobulin. The value for each individual protein can be raised if it can be isolates into a single and pure protein form. Individual whey protein hasnumerous potential applications in pharmaceutical, nutraceutical and food industry. In the current study, a series of chromatographic technique was used to recover the most abundant whey protein, β-lg from the whey. In the first step, whey was separated based on it size using size exclusion chromatography (SEC). Superdex200 and Superdex75 columns were tested for SEC experiment. Superdex75 showed better peak separation compared to the Superdex200 for whey fractionation. Further polishing step was done using 1 ml HiTrap Q-Sepharose anion exchange column to fully separate a-lac and β-lg protein fraction. Different linear salt elution gradient at 5 columns volume (CV) and 10 CV were studied. I 0 CV linear salt gradient protocol gives a better separation resolution between a-lac and β-lg mixture with some fraction contain mainly pure β-lg. Recovery of single β-lg from whey can added the value of the whey-based product and it can be used for specific application.

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of of Chemical Engineering) -- Universiti Malaysia Pahang - 2010, SV: DR SYED MOHD SAUFI BIN TUAN CHIK, NO CD: 5626
Uncontrolled Keywords: Protein Analysis; Chromatographic analysis; Whey
Subjects: Q Science > QD Chemistry
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Ms. Nurezzatul Akmal Salleh
Date Deposited: 31 May 2017 04:42
Last Modified: 31 May 2017 04:42
URI: http://umpir.ump.edu.my/id/eprint/17792
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