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Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology

Nur Syazana, Muhamad Nasrah and Mior Ahmad Khushairi, Mohd Zahari and N., Masngut and Hidayah, Ariffin (2017) Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology. In: MATEC Web of Conferences: Fluids and Chemical Engineering Conference (FluidsChE 2017) , 4-6 April 2017 , Kota Kinabalu, Sabah, Malaysia. pp. 1-8., 111 (03001). ISSN 2261-236X

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Abstract

The interaction between incubation temperature, yeast extract concentration and inoculum size was investigated to optimize critical environmental parameters for production of biobutanol from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824 using response surface methodology (RSM). A central composite design (CCD) was applied as the experimental design and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for the biobutanol production. The incubation temperature, yeast extract concentration and inoculum size showed significant value at p < 0.005. The results of optimization process showed that a maximum biobutanol production was obtained under the condition of temperature 37 °C, yeast extract concentration 5.5 g/L and inoculum size 10%. Under these optimized conditions, the highest biobutanol yield was 0.3054 g/g after 144 hours of incubation period. The model was validated by applying the optimized conditions and 0.2992 g/g biobutanol yield was obtained. These experimental findings were in close agreement with the model prediction, with a difference of only 9.76%.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Biobutanol; Response Surface Methodology; Central Composite Design; Oil Palm Frond Juice; Clostridium acetobutylicum (ATCC 824)
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 31 Oct 2017 04:07
Last Modified: 07 Mar 2018 01:52
URI: http://umpir.ump.edu.my/id/eprint/18685
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