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Improved peroxide biosensor based on Horseradish Peroxidase/Carbon Nanotube on a thiol-modified gold electrode

A. K. M., Kafi and Naqshabandi, M. and M. M., Yusoff and Crossley, Maxwell J. (2018) Improved peroxide biosensor based on Horseradish Peroxidase/Carbon Nanotube on a thiol-modified gold electrode. Enzyme and Microbial Technology, 113. pp. 67-74. ISSN 0141-0229

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Abstract

A new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface has been described in order to build up the effective electrical wiring of the enzyme units with the electrode. The synthesized 3D HRP/CNT network has been characterized with cyclic voltammetry and amperometry which results the establishment of direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the high biological activity and stability is exhibited by the immobilized HRP and a quasi-reversible redox peak of the redox centre of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficient α were found as 0.57 s−1 and 0.42, respectively. Excellent electrocatalytic activity for the reduction of H2O2 was exhibited by the developed biosensor. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0 × 10−7 to 1.2 × 10−4 M with a detection limit of 2.2.0 × 10−8 M at 3 σ. The Michaelies–Menten constant Kapp M value is estimated to be 0.19 mM. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Three-dimensional networks; Hrp; Carbon nanotube; Direct electron transfer; Biosensors Hydrogen peroxide
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Prof. Dr. Mashitah Mohd Yusoff
Date Deposited: 12 Jun 2018 02:35
Last Modified: 09 Apr 2019 03:47
URI: http://umpir.ump.edu.my/id/eprint/19746
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