UMP Institutional Repository

An integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah)

Noor Adilah, Md Salehan (2018) An integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah). Masters thesis, Universiti Malaysia Pahang.

[img]
Preview
Pdf
An integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah) - Table of contents.pdf - Accepted Version

Download (303kB) | Preview
[img]
Preview
Pdf
An integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah) - Abstract.pdf - Accepted Version

Download (105kB) | Preview
[img]
Preview
Pdf
An integrated ultrasonic-assisted enzymes as a single extraction unit for gallic acid from labisia pumila (kacip fatimah) - References.pdf - Accepted Version

Download (302kB) | Preview

Abstract

Labisia pumila (locally known as Kacip fatimah) extract contains phenolic compounds such as gallic acid that have multiple biological effects. Ultrasound-assisted extraction (UAE) is widely reported for the extraction of medicinal plants and herbs due to its economical and green technology. Therefore, this study applied UAE with unified enzymatic extraction (EnE) to enhance the extraction yield of gallic acid from Labisia pumila. The influence of five parameters on the extraction of gallic acid from Labisia pumila were investigated: extraction time (1–8 h), sample-to-solvent ratio (1:6, 1:8, 1:10, and 1:12 wt/wt), temperature (40, 50, 60, and 80 °C), sonication (10%, 20%, and 40% duty cycle), and cellulase concentration (0.025, 0.05, 0.1, 0.2, and 0.3 g/L). The power intensity of 8.66 W/cm2 was implemented using an ultrasonic processor Q700 (700 watts, 20 kHz). The results showed that the gallic acid yield was the highest at 50 °C with 1:10 sample-to-solvent ratio for aqueous extraction (AE) with 1.0251 ± 0.0569 mg/g of gallic acid extracted. Whereas, both ultrasound-assisted extraction (UAE) and enzymatic extraction (EnE) techniques gave the highest yield at 40% duty cycle and 0.05 g/L cellulase concentration with 1.8425 ± 0.1191 mg/g and 1.28565 ± 0.1760 mg/g gallic acid extracted, respectively. The result of the ultrasound-assisted enzymatic extraction (UEnE) indicated an increased trend when the extraction time was increased from 1 to 7 h. However, beyond 7 h the yield declined indicating that the degradation of gallic acid may have initiated. The highest gallic acid amount obtained from UAEnE was 2.9287 ± 0.4060 mg/g after 7 h of extraction with 2.91-fold increment compared with the AE result. Thus, the integrated single unit ultrasonic-assisted enzyme extraction successfully improved the gallic acid extraction from Labisia pumila.

Item Type: Thesis (Masters)
Additional Information: Thesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2018, SV: DR. AZILAH BINTI AJIT @ ABD AZIZ, NO. CD: 11598
Uncontrolled Keywords: Labisia pumila; gallic acid
Subjects: Q Science > QD Chemistry
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Sufarini Mohd Sudin
Date Deposited: 31 Dec 2018 04:14
Last Modified: 31 Dec 2018 04:14
URI: http://umpir.ump.edu.my/id/eprint/23262
Download Statistic: View Download Statistics

Actions (login required)

View Item View Item