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Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds

Batool, T. and Makky, Essam A. and M., Khan and M. M., Yusoff (2018) Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds. In: 4th International Conference Of Chemical Engineering And Industrial Biotechnology (ICCEIB 2018), 1-2 Aug 2018 , Seri Pacific Hotel, Kuala Lumpur. pp. 364-365..

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Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds.pdf

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Abstract

L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cultural parameters affecting the production of L-asparaginase were optimized [3]. Maximal yields of L-asparaginase were recorded from 5-day-old culture grown in nutrient broth in presence of Moringa Oleifera seeds as substrate with initial pH 7.0 at temperature 37◦C [4, 5]. Ammonium chloride (1%) and galactose (1%) served as good nitrogen and carbon sources for L-asparaginase production, respectively. Na+and K+ slightly enhanced the productivity of L-asparaginase [6]. The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme was also studied [7]. The enzyme showed a good scavenging activity against DPPH with IC50 value of 81.645µg/mL [8].

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: L-asparaginase; Bacillus sp; antitumor activity; Moringa olefiera
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 07 Jan 2019 08:20
Last Modified: 07 Jan 2019 08:20
URI: http://umpir.ump.edu.my/id/eprint/23639
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