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Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane

Natassha, Jamil and Rohaida, Che Man and Suhaily, Suhaimi and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2018) Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane. In: The 3rd International Conference on Green Chemical Engineering and Technology (3rd GCET), 7-8 November 2017 , Melaka, Malaysia. pp. 22036-22042., 5 (10). ISSN 2214-7853

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Abstract

Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been proven as a promising method to improve the enzyme stability in order to achieve the high production of CD. In this study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption. The effects of the enzyme concentration [0.6-1.4% (v/v)] and temperature (20-40 °C) on the immobilization yield were investigated. The highest immobilization yield of 80.0% was observed when 10% (v/v) of enzyme concentration was used. The optimal immobilization temperature obtained was at 25 °C with 95.6% of immobilization yield. Hence, the high immobilization yield contributed to the high production of CD which may be beneficial for the industrial purposes.

Item Type: Conference or Workshop Item (Lecture)
Additional Information: Indexed by SCOPUS
Uncontrolled Keywords: Adsorption; Cyclodextrin glucanotransferase; Enzyme concentration; Enzyme immobilization; Hollow fiber membrane; Temperature
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Ms. Rohaida Che Man
Date Deposited: 17 May 2019 04:00
Last Modified: 17 May 2019 04:00
URI: http://umpir.ump.edu.my/id/eprint/23883
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