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Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots

Rashid, Muhammad Abid and Akhtar, Muhammad Nadeem and Ashraf, Aisha and Nazir, Sadia and Ijaz, Anum and Noradila, Abdullah Omar and Noor, Sadia and Basit, Abdul and Tareen, Rasool Bakhsh (2018) Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots. Farmacia, 66 (1). pp. 165-171. ISSN 2065-0019

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In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (20.8%), p-cymene (20.9%), palmitic acid (19.2%) and vitamin E (13.8%) as major components of methanol, n-hexane, chloroform and ethyl acetate extracts respectively. The results of spectrophotometric assays showed that the methanol extract had the highest total phenolic contents (210.3 ± 0.74 μg GAE/mg of dry plant material), total flavonoid contents (75.5 ± 0.32 μg QE/mg of dry plant material) and antioxidant potential. It was established by disc diffusion and minimum inhibitory concentration methods that, overall, the methanol extract exhibited the highest antimicrobial activity against most of the tested microbial strains (Escherichia coli, Bacillus subtilis, Pasteurella multocida, Staphylococcus aureus, Aspergillus niger and Fusarium solani) as compared with the nhexane, chloroform, and ethyl acetate extracts. The outcome of the haemolytic assay showed that the tested extract and different solvent fractions were safe to human erythrocyte membrane

Item Type: Article
Subjects: Q Science > QR Microbiology
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Noorul Farina Arifin
Date Deposited: 23 Jan 2019 08:02
Last Modified: 23 Jan 2019 08:02
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