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Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay

Tsukada, Keiji and Tsunashima, Kenta and Jinno, Katsuya and Hiramatsu, Bunta and Takeuchi, Shun and Fujimoto, Kayo and Kenji, Sakai and Kiwa, Toshihiko and Mohd Mawardi, Saari (2019) Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay. IEEE Transactions on Magnetics, 55 (7). pp. 1-5. ISSN 0018-9464

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Abstract

The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not need bound/free marker separation and the ability to detect biological targets in a nebulous liquid. In addition, the wash-free process is very fast. In this paper, we tried to reduce the reaction time for the antigen-antibody reaction in order to make the MIA method more attractive. The reduction in reaction time and the detection characteristic of biological targets were demonstrated using C-reactive proteins (CRPs) as biological targets for diagnosing inflammation. To shorten the reaction time, a magnetic-shaking method using the magnetic field gradient from a neodymium magnet was developed. When comparing the reaction times with and without magnetic shaking, it was found that the reaction time decreased with magnetic shaking for all CRP concentrations. Even after the magnetic-shaking treatment, the dependence of the CRP concentration on the magnetic signal was observed. The number of surface modifications per magnetic marker and the magnetic marker concentration also affected the reaction time. In order to obtain the desired measurement range based on the CRP response characteristic, it is necessary to optimize the number of magnetic markers and polymer beads used.

Item Type: Article
Uncontrolled Keywords: Antigen-antibody reaction; C-reactive protein (CRP); magnetic field gradients; magnetic immunoassay (MIA); magnetic nanoparticles (MNPs)
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Faculty/Division: Faculty of Electrical & Electronic Engineering
Depositing User: Dr. Mohd Mawardi Saari
Date Deposited: 01 Jul 2019 04:54
Last Modified: 01 Jul 2019 04:54
URI: http://umpir.ump.edu.my/id/eprint/25077
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