Nurul Atikah, Mohd Rodzri and Salwani, Md Zain and Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and R. M., Illias (2019) Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3). Materials Today: Proceedings, 19 (4). pp. 1247-1254. ISSN 2214-7853. (Published)
|
Pdf
effect of pyhsical parameters.pdf Download (160kB) | Preview |
Abstract
ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered microorganism. In this study, recombinant E. coli BL21 (DE3) is used to produce ᴅ-xylonic acid from ᴅ-xylose and the effect of temperature, initial pH of medium and agitation rate on ᴅ-xylonic acid production is determined. Fermentation was carried out using super optimal broth medium in shake flasks for 24 hours. Results indicate that ᴅ-xylonic acid concentration was as high as 9.95 g L-1 with yield of 0.91 g/g ᴅ-xylose from 10 g/L when governed under temperature of 37°C, initial pH of medium pH 7 and agitation rate 200 rpm.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ᴅ-xylonic acid, recombinant E. coli BL21 (DE3), ᴅ-xylose, temperature |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering Institute of Postgraduate Studies |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 01 Oct 2019 04:27 |
| Last Modified: | 09 Feb 2021 08:45 |
| URI: | http://umpir.ump.edu.my/id/eprint/25949 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |