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Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

Hariharan, Ezhilarasu and Raghavendra, Ramalingam and Chetna, Dhand and Lakshminarayanan, Rajamani and Asif, Sadiq and Gandhimathi, Chinnasamy and Seeram, Ramakrishna and Boon, Huat Bay and Venugopal, Jayarama Reddy and Dinesh Kumar, Srinivasan (2019) Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. International Journal of Molecular Science, 20 (5174). pp. 1-16.

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Abstract

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-"-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc disffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.

Item Type: Article
Uncontrolled Keywords: Electrospinning; nanofibers; curcumin; aloe vera; tetracycline hydrochloride; fibroblasts; wound healing
Subjects: R Medicine > RL Dermatology
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Prof. Dr. Jayarama Reddy Venugopal
Date Deposited: 22 Oct 2020 07:25
Last Modified: 22 Oct 2020 07:25
URI: http://umpir.ump.edu.my/id/eprint/29674
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