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COVID-19 Outbreak in Malaysia: Decoding D614G Mutation of SARS-CoV-2 Virus Isolated from an Asymptomatic Case in Pahang

Aini Syahida, Mat Yassim and Mohd Fazli, Farida Asras and Ahmad Mahfuz, Gazali and Marcial-Coba, Martin S. and Ummu Afeera, Zainulabid and Hajar Fauzan, Ahmad (2021) COVID-19 Outbreak in Malaysia: Decoding D614G Mutation of SARS-CoV-2 Virus Isolated from an Asymptomatic Case in Pahang. Materials Today: Proceedings. pp. 1-9. ISSN 2214-7853 (In Press)

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SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus.

Item Type: Article
Uncontrolled Keywords: SARS-CoV-2, Mutation, D614G, Spike protein, Malaysia, Pahang
Subjects: Q Science > QR Microbiology > QR355 Virology
Faculty/Division: Centre of Excellence: Bio-Aromatic Research Center of Excellence
Faculty of Industrial Sciences And Technology
Depositing User: Dr. Hajar Fauzan Ahmad
Date Deposited: 11 Mar 2021 03:14
Last Modified: 11 Mar 2021 03:14
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