A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis

Aizi Nor Mazila, Ramli and Nur Dini, Johari and Mohd Akmal, Azhar and Rohaida, Che Man and Hazrulrizawati, Hamid (2021) A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis. Journal of Food Measurement and Characterization, 15. pp. 862-875. ISSN 2193-4126 (Print); 2193-4134 (Online). (Published)

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Abstract

A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the L-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of L-glutaminase from Kosakonia sp. with other L-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use.

Item Type:	Article
Uncontrolled Keywords:	L-glutaminase, Kosakonia radicincitans, 3D model
Subjects:	Q Science > Q Science (General)
Faculty/Division:	Faculty of Industrial Sciences And Technology
Depositing User:	Dr. Aizi Nor Mazila Ramli
Date Deposited:	12 May 2022 02:37
Last Modified:	12 May 2022 02:37
URI:	http://umpir.ump.edu.my/id/eprint/34064
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