Patricia, Vit and Bajaree, Chuttong and Norhasnida, Zawawi and Maria, Diaz and Jane Van, der Meulen and Hajar F., Ahmad and Francisco A., Tomas-Barberan and Gina, Meccia and Khanchai, Danmek and J. Enrique, Moreno and David W., Roubik and Ortrud Monika, Barth and Dirk W., Lachenmeier and Michael S., Engel (2022) A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest. Sociobiology, 69 (4). e8251. ISSN 0361-6525. (Published)
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Abstract
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | antioxidant activity; biosurfactant activity; 1H-NMR; flavonoids; Meliponini; melyssopalinology; sensory analysis |
| Subjects: | H Social Sciences > HD Industries. Land use. Labor Q Science > Q Science (General) |
| Faculty/Division: | Faculty of Industrial Sciences And Technology |
| Depositing User: | Dr. Hajar Fauzan Ahmad |
| Date Deposited: | 26 Jul 2023 05:08 |
| Last Modified: | 26 Jul 2023 05:12 |
| URI: | http://umpir.ump.edu.my/id/eprint/38075 |
| Download Statistic: | View Download Statistics |
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