Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia

Dullah, Elvina Clarie and Mohd Fazli, Farida Asras (2023) Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia. Makara Journal of Technology, 27 (3). pp. 93-98. ISSN 2355-2786. (Published)

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Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, and catalase tests, while negative results were obtained from Voges-Proskauer (VP) and indole tests. Three different caffeine concentrations of 0.25%, 0.4%, and 2% were tested and measured through Gas Chromatography-Mass Spectrophotometry (GC-MS) analysis. The highest caffeine reduction (89.25%) was found when 0.25% caffeine was used in the media. Only a small amount of caffeine was reduced to 0.4% and 2%, with 34.78% and 46.16%, respectively. Under microscopic observation, the shape of the isolated bacteria was rod bacilli and was stained with a pink color, which indicates Gram-negative bacteria. Comparing the results with previous research and observing the color of the pure culture, which revealed a yellow color, bacteria isolated from the coffee plantation area can be concluded to be a partially identified Pseudomonas sp.

Item Type: Article
Uncontrolled Keywords: Caffeine-degrading; Coffee; Pseudomonas
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 16 Feb 2024 07:59
Last Modified: 16 Feb 2024 07:59
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