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Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation

Syed Mohd Saufi, Tuan Chik and Fee, Conan J. (2013) Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation. Journal of Membrane Science, 444. pp. 157-163. ISSN 0376-7388

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Abstract

The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column.

Item Type: Article
Uncontrolled Keywords: Membrane chromatography; Hydrophobic interaction; Whey protein; Mixed matrix membrane
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 27 Aug 2015 03:10
Last Modified: 11 Apr 2018 01:57
URI: http://umpir.ump.edu.my/id/eprint/6215
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