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Enhanced production of extracellular urate oxidase by Aspergillus Flavus

Wan Ruhayu, Wan Ramli (2013) Enhanced production of extracellular urate oxidase by Aspergillus Flavus. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.


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Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillusfiavus. The parameters studied were pH (range of 4 to 8), the sucrose concentration (lOg/i to 50 Wi) and the agitation rate (100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30°C. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration. Since the optimum conditions were obtained, the production of uricase can be enhanced and world demand on uricase can be fulfill in the future.

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
Uncontrolled Keywords: Enzymes Therapeutic use Uric acid Oxidation Aspergillus
Subjects: R Medicine > RM Therapeutics. Pharmacology
Depositing User: nurudin nasir
Date Deposited: 09 Dec 2014 07:59
Last Modified: 08 Jun 2021 06:50
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