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Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties

Kuppusamy, Palaniselvam and M. M., Yusoff and Narasimha, Reddy Parine and Solachuddin J.A., Ichwan and Gaanty Pragas, Maniam and Natanamurugaraj, Govindan (2015) Intracellular Biosynthesis of Au and Ag Nanoparticles using Ethanolic Extract of Brassica oleracea L. and Studies on their Physicochemical and Biological Properties. Journal of Environmental Sciences, 29. pp. 151-157. ISSN 1001-0742

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Abstract

In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles ( NPs) in green chemistry method . The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer , Field Emission Scanning Electron Micros copy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanop articles were seen in spherical shapes. The average spherical size of the particles was observed in 24– 38 nm for gold and 30–45 nm for silver NPs . X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally , the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes ( sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/mL respectively. Inaddition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens.

Item Type: Article
Uncontrolled Keywords: Biosynthesis; Broccoli extract; Gold; Silver nanoparticles; Antimicrobial
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Suhara
Date Deposited: 04 Jun 2015 04:52
Last Modified: 27 Jul 2018 01:33
URI: http://umpir.ump.edu.my/id/eprint/8923
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