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Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

Wan Siti Atikah, Wan Omar and Ahmad Ziad, Sulaiman and Azilah, Ajit and Yusuf, Chisti and Mohd Hairul, Ab Rahim and Adam Leow, Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 71-76..

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Abstract

Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: transformation, Escherichia coli, recombinant, lipase, plasmid
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Industrial Sciences And Technology
Depositing User: Noorul Farina Arifin
Date Deposited: 26 Oct 2016 07:28
Last Modified: 16 Aug 2017 02:25
URI: http://umpir.ump.edu.my/id/eprint/15042
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