UMP Institutional Repository

Production of arachidonic acid from candida krusei

Nurul Izyani, Abdul Manan (2012) Production of arachidonic acid from candida krusei. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.

[img]
Preview
PDF (Production of arachidonic acid from candida krusei-Table of contents)
Production of arachidonic acid from candida krusei - Table of contents.pdf - Accepted Version

Download (143kB) | Preview
[img]
Preview
PDF (Production of arachidonic acid from candida krusei-Abstract)
Production of arachidonic acid from candida krusei - Abstract.pdf - Accepted Version

Download (141kB) | Preview
[img]
Preview
PDF (Production of arachidonic acid from candida krusei-Chapter 1)
Production of arachidonic acid from candida krusei - Chapter 1.pdf - Accepted Version

Download (144kB) | Preview
[img]
Preview
PDF (Production of arachidonic acid from candida krusei-References)
Production of arachidonic acid from candida krusei - References.pdf - Accepted Version

Download (190kB) | Preview

Abstract

The potential of arachidonic acid (AA) in pharmaceutical field had been discovered for a long time. AA is known as a fatty acid that can help information transfer of nerve systems, other than involves in growth and repair of skeletal muscle tissue. Microorganisms such as yeasts are one of the sources that can be commercialized as AA producer. By using microorganisms, the production process become less complicated compared to using animal source, and the ‘Halal’ standard can be fixed. The works done in this research investigate the optimum condition for AA production from yeast. In this research, the best culture condition that produced the highest yield of AA was determined by varying the pH, the carbon source and the agitation speed. The range of pH is 5 to 9. The carbon sources that were used are sucrose, starch and the mixture of sucrose and starch. Speed of incubator shaker was set at 50 rpm, 150 rpm and 250 rpm. Extraction of lipid was done by using twostep Bligh and Dyer (1959) method. Chloroform and methanol were added in specific ratio before centrifugation. The lower phase which contain lipid was collected. After evaporation, the lipid extract was dissolved in small volume of chloroform and methanol. The lipids obtained were analyzed in gas chromatography. The highest dry biomass concentration was produced at a culture at pH 7 and 250 rpm agitation rate by using sucrose while the highest yield of AA was produced at pH 7 and 150 rpm agitation rate by using sucrose as carbon source. AA production from yeast had shown advantages over other sources. Further studies on optimization of AA production should be directed at varying other significant parameters including nutrients needed and method of extraction. The findings in this study will be helpful for other research about AA in the future.

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2012, SV: NORAZIAH ABU YAZID, NO CD: 6377
Uncontrolled Keywords: Yeast fungi Biotechnology; Arachidonic acid
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Ms. Nurezzatul Akmal Salleh
Date Deposited: 12 May 2017 08:12
Last Modified: 12 May 2017 08:12
URI: http://umpir.ump.edu.my/id/eprint/17687
Download Statistic: View Download Statistics

Actions (login required)

View Item View Item