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A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study

A. K. M., Kafi and M. M., Yusoff and Choucair, Mohammad and Crossley, Maxwell J. (2017) A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study. Journal of Solid State Electrochemistry, 21 (10). pp. 2761-2767. ISSN 1433-0768

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The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt c and the modified graphene-based electrode was established. The modified electrode was employed as a high-performance hydrogen peroxide (H2O2) biosensor. The Cyt c present in modified electrode exhibited a pair of quasi-reversible redox peaks with a midpoint potential of −0.380 and −0.2 V, cathodic and anodic, respectively. Investigations into the electrocatalytic activity of the modified electrode upon hydrogen peroxide exposure revealed a rapid amperometric response (5 s). Under optimized conditions, the linear range of response to H2O2 concentration ranged from 5 × 10−7 to 2 × 10−4 M with a detection limit of 2 × 10−7 M at a signal-to-noise ratio of 3. The stability, reproducibility, and selectivity of the proposed biosensor are discussed in relation to the morphology and composition of the modified electrode.

Item Type: Article
Uncontrolled Keywords: Graphene electrode; Crosslinked networks; Cytochrome C; Direct electrochemistry; Biosensor
Subjects: Q Science > Q Science (General)
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 06 Nov 2017 07:54
Last Modified: 26 Jul 2018 03:20
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