Mahmood, Syed and Sengupta, Pinaki and Mandal, Uttam K. and Chatterjee, Bappaditya and Taher, Muhammad (2017) Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma. Latin American Journal of Pharmacy, 36 (9). pp. 1901-1908. ISSN 2362-3853. (Published)
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Abstract
SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific and stable. The applicability of the method was established through a single dose oral pharmacokinetic study of raloxifene in rat.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed in Scopus and ISI |
| Uncontrolled Keywords: | bioanalytical method development; pharmacokinetic application; raloxifene hydrochloride; rat plasma; validation. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Faculty/Division: | Faculty of Engineering Technology |
| Depositing User: | Dr. Syed Mahmood |
| Date Deposited: | 05 Feb 2018 02:31 |
| Last Modified: | 05 Feb 2018 02:31 |
| URI: | http://umpir.ump.edu.my/id/eprint/19791 |
| Download Statistic: | View Download Statistics |
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