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Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal

Abdul Aziz, Mohd Azoddein and Siti Nur Baizura, Yusuf and Faten Ahada, Mohd Azli and Ahmad Bazli, Bustary and Mariah, Che Mamat and Nur Athirah, Mohammad Basir (2018) Study of enzyme purification method and growth pattern for Pseudomonas putida in mercury removal. In: 5th International Conference On “Trends In Multidisciplinary Business & Economic Research” (TMBER -2018), 23-24 March 2018 , Rydges Camperdown, Sydney Australia. pp. 1-6.. (Unpublished)

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Abstract

Mercury is one of the toxic element and very harmful. The exposure of the mercury will mainly cause health effect and the exposure can be in term of dose, age of person exposed, duration exposed route exposed and duration of exposed. In Malaysia, Mercury is one of the heavy metals of concern, found in wastewaters coming from oil refinery and petrochemical industries. Mercury and mercurial compounds are highly toxic contaminants in the aquatic systems and soils. The proper disposal of wastewater should be implement with the Department of Environment of Malaysia (DOE) which is the concentration of mercury should be 0.05 ppm for standard B in wastewater for the sake of people and environment health. There are many types of mercury removal technology to reduce the concentration of mercury such as chemical treatment, ion exchange, membrane filtration, adsorption as well as bioremediation which used for mercury removal in this study. This paper present the activity of enzyme from Pseudomonas putida (P. putida) is produced in the mercury treatment from petrochemical wastewater at optimum condition. P. putida growth at optimum condition in mercury different concentration was observed. Conclusion optimum condition concentration of mercury and shaker speed at 7 ppm is 41 ˚C and 180 rpm respectively, with able to reduce 92.59 % mercury concentration. During the processes of mercury removal, mercuric reductase was produced at value of 56 kDa.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Enzyme purification; Growth pattern; P. putida; Mercury removal
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 29 Jun 2018 03:57
Last Modified: 29 Jun 2018 03:57
URI: http://umpir.ump.edu.my/id/eprint/21401
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