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Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata

Nang Nor Azimah, Long Nadzri and Mior Ahmad Khushairi, Mohd Zahari and Saiful Nizam, Tajuddin and Che Mohd Aizal, Che Mohd (2018) Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata. Malaysian Applied Biology, 47 (2). pp. 9-13. ISSN 0126-8643

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Abstract

Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration of glucose (g/L) on the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate from OPF juice by using Ceratocystis fimbriata (C. fimbriata). The design of experiment (DOE) method was used to screen the best parameters affecting the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The Gas Chromatography-Mass Spectroscopy (GC-MS) with Solid Phase Micro Extraction (SPME) was used to analyze and separate the peak of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. Based on the GC-MS analysis, results showed that the most favorable condition for methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate production was achieved at an initial pH medium (8), agitation speed (100 rpm), temperature (25°C) and 30 g/L of glucose in OPF juice. Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate were produced when the retention time was 32.80 min, the relative peak area was 0.24 % of chromatogram area. This result showed the great potential usage of OPF juice as a substrate to produce methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate by C. fimbriata.

Item Type: Article
Uncontrolled Keywords: Oil palm frond juice, Methyl 3-(3,5-di-tert-butyl 4-hydroxyphenyl) propionate, Ceratocystis fimbriata
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Noorul Farina Arifin
Date Deposited: 24 Jul 2018 08:18
Last Modified: 24 Jul 2018 08:18
URI: http://umpir.ump.edu.my/id/eprint/21772
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