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Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool

Alara, Oluwaseun Ruth and Nour, A. H. and Olalere, Olusegun Abayomi (2018) Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization, 12 (2). pp. 1107-1122. ISSN 2193-4126 (Print); 2193-4134 (Online)

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Abstract

In this study, ethanolic microwave-assisted extraction of total phenolic content (TFC), total flavonoid content (TFC) and antioxidant from Vernonia amygdalina leaf was investigated using response surface methodology. Four extraction variables, including irradiation time (1‒5 min), microwave power level (500‒700 W), feed-to-solvent ratio (1:8‒1:12 g/ml), and ethanol concentration (60‒80%) were optimized to obtain optimal yields. The analysis of variance results showed that ethanol concentration, microwave power level and irradiation time mostly affected the TFC, TPC and antioxidant activities significantly (p < 0.05), whereas the feed-to-solvent ratio was insignificant. The validated optimal yields of TPC (113.76 mg GAE/g d.w.), TFC (94.08 mg QE/g d.w.), DPPH (97.98%), and ABTS (99.34%) were obtained at extraction conditions, viz, 4 min of irradiation time, 558 W of microwave power, feed-to-solvent ratio of 1:10 g/ml, and ethanol concentration of 76%. In addition, the phytochemical profiling of the extract at optimal conditions confirmed the presence of phenolic and flavonoid compounds.

Item Type: Article
Uncontrolled Keywords: Response surface methodology; Phenols; Flavonoids V.; amygdalina leaf; Antioxidant activities
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Prof. Dr. Abdurahman Nour
Date Deposited: 09 Aug 2018 04:49
Last Modified: 08 Jan 2019 05:40
URI: http://umpir.ump.edu.my/id/eprint/21926
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