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Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique

Alara, Oluwaseun Ruth and Nour, A. H. and Ukaegbu, Chinonso Ishmael and Azhari, H. Nour (2018) Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique. Industrial Crops and Products, 122. pp. 533-544. ISSN 0926-6690

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Abstract

Diabetes mellitus is a metabolic disorder disease emanating from the inability of body’s response to high glucose levels in the blood. However, the side-effects from consuming synthetic drugs have reduced the effectiveness, thus, the potential of purple fleabane (Vernonia cinerea) leaf was investigated using microwave-assisted extraction (MAE) in this study. The influences of extraction factors such as irradiation time, microwave power level, feed/solvent, and ethanol concentration on the recovery yields of polyphenol and flavonoids were as well studied. Thirty experimental runs using a face-centered central composite design (FCCCD) with six centre points were employed and optimal yields were obtained by generating quadratic models based on response surface methodology. The obtained optimal MAE conditions were as follows: irradiation time, 2 min; microwave power level, 444 W; feed/solvent, 1:14 g/mL; and ethanol concentration, 47% v/v. There was an insignificant difference between the predicted and experimental data (p > 0.05), showing good predictive models for the extraction of phenolic compounds from V. cinerea leaves using MAE. Also, the results confirmed that MAE can recover higher phenolic compounds, antioxidant, and anti-diabetic activities from V. cinerea leaves in shorter extraction time. In addition, the phenolic compounds in V. cinerea leaf extract were identified using liquid chromatography-mass spectrometry quadrupole time of flight (LC–MS-Q-TOF) and Fourier transform infrared spectroscopy (FTIR). The extracts showed higher anti-diabetic activity using alpha-amylase and alpha-glycosidase assays which suggested its exploration in pharmaceutical and functional food industries

Item Type: Article
Uncontrolled Keywords: Vernonia cinerea leaf; Phenolic compounds; Antioxidant; Response surface methodology; Microwave-assisted extraction Anti-diabetic activity
Subjects: T Technology > TP Chemical technology
Faculty/Division: Centre of Excellence: Centre of Excellence for Advanced Research in Fluid Flow
Faculty of Industrial Sciences And Technology
Depositing User: Prof. Dr. Abdurahman Nour
Date Deposited: 09 Aug 2018 06:27
Last Modified: 14 Jan 2019 00:59
URI: http://umpir.ump.edu.my/id/eprint/21930
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