Jaya Vejayan, Palliah and Aini Norhidayah, Mohamed and Amira Alia, Zulkifli and Yasmin Amira, Che Yahya and Norliana, Munir and M. M., Yusoff (2018) Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products. Current Science, 115 (5). pp. 886-894. ISSN 0011-3891. (Published)
|
Pdf
Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products.pdf Download (263kB) | Preview |
|
![[img]](http://umpir.ump.edu.my/style/images/fileicons/other.png) |
Pdf
Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products.pdf Restricted to Repository staff only Download (1MB) | Request a copy |
Abstract
The benefits of Eurycoma longifolia (Tongkat Ali, TA) for its aphrodisiac capabilities are well known and many products are marketed worldwide. Due to its popularity, the plant is being abused for promoting fake products. Therefore, there is a need for better testing of the markers required by authorities and responsible manufacturers. A low-molecular-weight protein has been studied for developing it as a testing marker. Two dimensional electrophoresis (2DE) (four spots were observed) was used for positive detection of proteins in an aqueous extract of TA root and the pronounced separation of a Coomassie-stained spot, subsequently referred to as Marker A. Consecutive chromatographic separations of the aqueous extract of TA led to the isolation of pure protein from Marker A. When this marker was used to test 46 TA-based products randomly selected from markets worldwide, in 20 of them, the results were found to be comparable to those obtained using the organic eurycomanone marker. The ranking of products from the highest quantity to the lowest observed to be ordered differently if compared for both markers. This is an expected outcome because Marker A was measured for its protein content and eurycomanone for its organic molecule. Marker A detection using 2DE is shown to be a useful tool to test products supplemented with E. longifolia root.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Authentication; Electrophoresis; Eurycoma longifolia; Herbal products; Markers |
| Subjects: | Q Science > Q Science (General) |
| Faculty/Division: | Faculty of Industrial Sciences And Technology |
| Depositing User: | Mrs. Neng Sury Sulaiman |
| Date Deposited: | 03 Dec 2018 04:26 |
| Last Modified: | 03 Dec 2018 04:26 |
| URI: | http://umpir.ump.edu.my/id/eprint/22572 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |