Raja Zaidatul Akhmar, Raja Jamaluddin and Heng, Lee Yook and Tan, Ling Ling and K. F., Chong (2018) Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin. Sensors, 18 (5). pp. 1-17. ISSN 1424-8220. (Published)
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Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin.pdf Download (3MB) | Preview |
Abstract
A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | edible bird’s nest, electrochemical biosensor, graphene, hemoglobin, nitrite |
| Subjects: | Q Science > Q Science (General) |
| Faculty/Division: | Faculty of Industrial Sciences And Technology |
| Depositing User: | Mrs. Neng Sury Sulaiman |
| Date Deposited: | 15 Jan 2019 04:47 |
| Last Modified: | 15 Jan 2019 04:47 |
| URI: | http://umpir.ump.edu.my/id/eprint/23075 |
| Download Statistic: | View Download Statistics |
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