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Parametric study of soxhlet extraction of phenolic compounds from vernonia amygdalina leaves

Alara, Oluwaseun Ruth and Nour, A. H. and Siti Kholijah, Abdul Mudalip and Ukaegbu, Chinonso Ishmael and Azhari, H. Nour and Kabbashi, Nassereldeen Ahmed (2018) Parametric study of soxhlet extraction of phenolic compounds from vernonia amygdalina leaves. In: Proceedings Book: National Conference for Postgraduate Research (NCON-PGR 2018), 28-29 August 2018 , Universiti Malaysia Pahang, Gambang, Pahang. pp. 213-219.. ISBN 978-967-22260-5-5

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Abstract

Phenolic compounds are important secondary metabolites with different pharmacological activities. The effects of extraction parameters of Soxhlet extraction method which include extraction time, solid-to-solvent ratio and ethanol concentration on the recoveries of extraction yields, total phenolic content (TPC) and total flavonoid content (TFC) from extracts of Vernonia amygdalina leaves were investigated in this study using one-factor-at-a-time experiment (OFAT). Then, the functional group characterization of the extracts at maximum conditions was carried out. The results obtained showed that the three considered extraction parameters significantly influence the recoveries of extraction yield, TPC and TFC. Highest recoveries of extraction yield (14.22±0.19% w/w), TPC (75.94±2.15 mg GAE/g d.w.) and TFC (46.53±1.14 mg QE/g d.w.) were obtained at extraction time of 2 h, solid-to-solvent ratio of 1:20 g/mL and ethanol concentration of 60% v/v. Moreover, 214 National Conference for Postgraduate Research (NCON-PGR) six major peaks were identified from the Soxhlet extraction of V. amygdalina leaves at maximum conditions using Fourier transform infrared spectroscopy (FTIR) analysis. Thus, this study showed that V. amygdalina leaf is a potential source of phenolic compounds which can be influence by the extraction parameters.

Item Type: Conference or Workshop Item (Speech)
Uncontrolled Keywords: Vernonia amygdalina; Soxhlet extraction; Total phenolic content; Total flavonoid content
Subjects: T Technology > TP Chemical technology
Faculty/Division: Centre of Excellence: Centre of Excellence for Advanced Research in Fluid Flow
Faculty of Chemical & Natural Resources Engineering
Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Pn. Hazlinda Abd Rahman
Date Deposited: 22 Apr 2019 03:51
Last Modified: 28 Feb 2020 08:12
URI: http://umpir.ump.edu.my/id/eprint/23089
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