UMP Institutional Repository

Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques

Alara, Oluwaseun Ruth and Nour, A. H. and Ishmael, Ukaegbu Chinonso and Kabbashi, Nassereldeen Ahmed (2019) Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science, 13 (1). pp. 414-422. ISSN 1658-3655

[img]
Preview
Pdf
Extraction and characterization of bioactive compounds in Vernonia.pdf
Available under License Creative Commons Attribution Non-commercial.

Download (2MB) | Preview

Abstract

The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique.

Item Type: Article
Additional Information: Indexed by Web of Science
Uncontrolled Keywords: Vernonia amygdalina leaf; Extraction; Phytochemicals; Scanning electron microscopy; Gas chromatography-mass spectroscopy
Subjects: Q Science > QD Chemistry
Q Science > QK Botany
T Technology > TP Chemical technology
Faculty/Division: Centre of Excellence: Centre of Excellence for Advanced Research in Fluid Flow
Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 10 Jun 2019 03:38
Last Modified: 21 Feb 2020 07:34
URI: http://umpir.ump.edu.my/id/eprint/24723
Download Statistic: View Download Statistics

Actions (login required)

View Item View Item