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Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine

Pourjavaheri, Firoozeh and Ostovar Pour, Saeideh and Jones, Oliver A. H. and Smooker, Peter M. and Brkljača, Robert and Sherkat, Frank and Blanch, Ewan W. and Gupta, Arun and Shanks, Robert A. (2019) Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine. Process Biochemistry, 82. pp. 205-214. ISSN 1359-5113

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Abstract

Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α-helix and ß-sheet based keratin could be extracted from CF using sodium sulfide and L-cysteine with high yields. This is the first report of CF keratin extraction using L-cysteine.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Feathers; Green chemistry; Protein; Polymers
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 24 Oct 2019 06:41
Last Modified: 24 Oct 2019 06:41
URI: http://umpir.ump.edu.my/id/eprint/25071
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