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Comparative evaluation of protease extraction from leaves of syzygium polyanthum

Noorhafiza, Yahya and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Rohaida, Che Man and Shalyda, Md Shaarani and Mohd Hairul, Ab. Rahim (2019) Comparative evaluation of protease extraction from leaves of syzygium polyanthum. Indonesian Journal of Chemistry, 19 (2). pp. 503-510. ISSN 1411-9420 (Print); 2460-1578 (Online)

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Abstract

In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods.

Item Type: Article
Additional Information: Indexed by WOS & Scopus
Uncontrolled Keywords: Plant protease; Protease extraction; Syzygium polyanthum
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Industrial Sciences And Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 25 Oct 2019 03:32
Last Modified: 25 Oct 2019 03:32
URI: http://umpir.ump.edu.my/id/eprint/25336
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