Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology

Zatul Iffah, Mohd Arshad and Azura, Amid (2018) Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology. In: The 4th International Conference On The Applications Of Science And Mathematics 2018 , 13 - 14 Ogos 2018 , Tunku Tun Aminah Library UTHM, Parit Raja, Johor. pp. 377-382., 7 (4.30).

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Abstract

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

Item Type:	Conference or Workshop Item (Other)
Uncontrolled Keywords:	Aqueous Two-Phase System (ATPS); Face-Centered Central Composite Design (FCCCD); Optimization; Response Surface Methodology (RSM); Recombinant bromelain
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering
Depositing User:	Pn. Hazlinda Abd Rahman
Date Deposited:	21 Nov 2019 03:24
Last Modified:	21 Nov 2019 03:24
URI:	http://umpir.ump.edu.my/id/eprint/25502
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