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Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles

Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad (2019) Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles. Indonesian Journal of Chemistry, 19 (3). pp. 737-746. ISSN 1411-9420 (Print); 2460-1578 (Online)

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Abstract

This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.

Item Type: Article
Additional Information: Indexed by WOS
Uncontrolled Keywords: Reverse micelles; Sodium bis(2-ethylhexyl) sulfosuccinate (AOT); Jacalin; Forward extraction efficiency (FEE)
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Engineering Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 22 Nov 2019 04:09
Last Modified: 22 Nov 2019 04:09
URI: http://umpir.ump.edu.my/id/eprint/25736
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