Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and Nurul Atikah, Mohd Rodzri and Mior Ahmad Khushairi, Mohd Zahari and Wan Salwanis, Wan Md. Zain (2019) Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3). Materials Today: Proceedings, 19 (Part 4). pp. 1467-1472. ISSN 2214-7853. (Published)
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Abstract
Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 °C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Xylonic acid, Recombinant Escherichia coli, Xylose, SOB Medium |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 01 Oct 2019 02:30 |
| Last Modified: | 09 Feb 2021 02:34 |
| URI: | http://umpir.ump.edu.my/id/eprint/25948 |
| Download Statistic: | View Download Statistics |
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