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Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing

A. K. M., Kafi and Alim, Samiul and Rajan, Jose and M. M., Yusoff (2019) Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing. Journal of Electroanalytical Chemistry, 852 (113550). pp. 1-6. ISSN 1572-6657

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Abstract

Herein, we propose a new approach to fabricate an enzyme electrode based on immobilization of glucose oxidase (GOD) with multiporous nanofibers (MPNFs) of SnO2 on Prussian blue (PB) modified gold (Au) electrode with chitosan. MPNFs of SnO2 were synthesized by electrospinning method from the tin. Prussian blue was electrochemically deposited onto an Au electrode. GOD was then co-immobilized with the nanofibers on the surface of the PB modified gold electrode by using chitosan. The immobilized GOD/MPNFs onto the PB-Au presented faster, more stable and sensitive amperometric response to detecting glucose. Hydrogen peroxide (H2O2) produced via Glucose oxidation and glucose reaction was detected by Prussian blue. The linear response dependence on glucose concentration was observed in a range of 0.5–5 mM and a detection limit of 0.05 mM at 3 signal/noise ratio. Because of unique structural characteristics at the nanoscale level, multiporous nanofibers (MPNFs) of SnO2 is an ideal candidate for matrices in biosensing.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Multiporous SnO2 nanofiber; Prussian blue; Glucose oxidase; Glucose biosensor
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 05 Mar 2020 09:00
Last Modified: 05 Mar 2020 09:00
URI: http://umpir.ump.edu.my/id/eprint/26719
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