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Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane

Arumugam, Gayathri (2010) Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.

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Abstract

Separation of whey protein in goat milk by cross flow filtration using hollow fibre membrane is a good way for protein purification Cross flow filtration is a good protein purification method because it can operate continuously and does not require frequent maintenance therefore can be applied for industrial use . Protein separation by microfiltration requires pretreatment which includes centrifugation and filtration using Whatman filter paper to remove fat layer . Casein precipitation is done by reducing pH of goat milk to 4.6 to avoid fouling la yer on membrane . Microfiltration is rim using whey solution of varying pH and cross flow velocity to stud y concentration of whey protein in permeate during cross flow filtration . Whey protein concentration in sample collected froth permeate is analysed using Reverse Phase Chromatograpy RPC) where peak obtained is compared with standard to determine whey concentration . pH values selected is from range 6 to 8 and cross flow velocity range from 0.01 cm/s to 0.03 cm/s .. Concentration of whey protein B SA and f - lactoglobulin shows highest value compared to other pH values . pH 6.5 was an optimum pH because .wheyprotein gets denatured at higher pH and gets precipitated at lower pH . Concentration of whey protein is highest at cross flow velocity value of 0.03 cm/s . Whe y protein concentration increases steadilyas cross flow velocity increases . Cross flow filtration is an efficient method for protein purification and can be applied in industry.

Item Type: Undergraduates Project Papers
Uncontrolled Keywords: Proteins - Separation Separation (Technology) Ultrafiltration
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Shamsor Masra Othman
Date Deposited: 04 Dec 2012 03:35
Last Modified: 03 Mar 2015 07:57
URI: http://umpir.ump.edu.my/id/eprint/2694
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