Uric acid detection using uv-vis spectrometer

Nor Mazlee, Norazmi and Zul Rasyied, Abdul Rasat and Mazuina, Mohamad and Hadi, Manap (2017) Uric acid detection using uv-vis spectrometer. In: IOP Conference Series: Materials Science and Engineering, 4th International Conference on Mechanical Engineering Research (ICMER2017) , 1-2 August 2017 , Kuantan, Pahang, Malaysia. pp. 1-6., 257 (012031). ISSN 1757-899X

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Abstract

The aim of this research is to detect uric acid (UA) concentration using Ultraviolet-Visible (UV-Vis) spectrometer in the Ultraviolet (UV) region. Absorption technique was proposed to detect different uric acid concentrations and its UV absorption wavelength. Current practices commonly take a lot of times or require complicated structures for the detection process. By this proposed spectroscopic technique, every concentration can be detected and interpreted into an absorbance value at a constant wavelength peak in the UV region. This is due to the chemical characteristics belong to the uric acid since it has a particular absorption cross-section, σ which can be calculated using Beer’s Lambert law formula. The detection performance was displayed using Spectrasuite sofware. It showed fast time response about 3 seconds. The experiment proved that the concentrations of uric acid were successfully detected using UV-Vis pectrometer at a constant absorption UV wavelength, 294.46 nm in a low time response. Even by an artificial sample of uric acid, it successfully displayed a close value as the ones reported with the use of the medical sample. It is applicable in the medical field and can be implemented in the future for earlier detection of abnormal concentration of uric acid.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Uric Acid (UA); Ultraviolet-Visible (UV-Vis); Characteristics; Concentrations
Subjects: T Technology > T Technology (General)
Faculty/Division: Faculty of Engineering Technology
Depositing User: Pn. Hazlinda Abd Rahman
Date Deposited: 16 Jan 2020 03:14
Last Modified: 16 Jan 2020 03:20
URI: http://umpir.ump.edu.my/id/eprint/27261
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