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Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia

M. K. K., Fazlul and Y., Farzana and A., Najnin and M. A., Rashid and M. H. M., Nazmul (2019) Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia. Baghdad Science Journal, 16 (3). pp. 682-688. ISSN 2078-8665

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Abstract

The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL producing E. coli against antibiotics belonging to different families showed the highest resistance rates to Ampicillin (100%), Cefotaxime (97%), Cefuroxime (95%), and Ciprofoxacin (86%). Carbapenem groups of antibiotics, Meropenem (89%) and Imipenem (85%) have the highest susceptibility rate among all antibiotics used in this study. The outcome of the antimicrobial susceptibility testing of significant CTX-M- type ESBL producing E. coli could be useful to avoid failure or prolong treatments.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: CTX-M gene; ESBL; Escherichia coli; PCR
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
R Medicine > RA Public aspects of medicine
T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 31 Mar 2020 23:53
Last Modified: 31 Mar 2020 23:53
URI: http://umpir.ump.edu.my/id/eprint/27552
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